Comparison between unbuffered and buffered formalin fixatives for estrogen receptor and Ki-67 immunostains in breast cancer specimens: a pilot study

Pre-analytic steps are important for the immunohistochemical study as in the case of breast cancers. The initial process is the fixation in which the 10% neutral buffered formalin (NBF) is recommended. 10% formalin is less expensive and easier to prepare but its effect on the immunostaining is controversial....