immunohistochemistry

ALK-positive large B-cell lymphoma (ALK-positive LBCL) is a rare type of lymphoma. Because of the overlap of morphological and immunophenotypic features with other haematologic and non-haematologic neoplasms, diagnosing ALK-positive LBCL can be challenging. The lymph nodes showed a marked diffuse infiltrate of monomorphic large immunoblast-like cells with round pale nuclei containing a large central nucleolus and abundant amphophilic cytoplasm. Lymphoma cells were strongly positive for the ALK protein. ...

Tropomyosin receptor kinase (TRK) gene fusion has been found in associated with many tumours and coold be targeted treatment. Immunohistochemistry (IHC) expression of TRK is widely used to screening this alternation. The aim of this study was to investigate the expression of TRK protein detected by IHC in Thai cholangiocarcinoma (CCA) and to correlate with clinicopathological and survival data. Retrospective study of CCA patients who diagnosed from January 2011 to December 2015. ...

Several epigenetic mechanisms of oncogenesis such as histone acetylation and DNA methylation are now known to play a key role in developing cancers. So far, these molecolar events have rarely been evaluated in thyroid neoplasms. The aim of this study was to estimate the histone acetylation level in benign and malignant thyroid lesions and compare it to the normal counterpart....

The equivocal range of HER2 immunohistochemistry (IHC) is complete weak to moderate staining > 10% of breast cancer cells. This range has increased the numbers of HER2 in situ hybridisation (ISH) testing compared to the previous recommendations. ...

Gold standard of HER2 amplification detection is fluorescence in situ hybridisation (FISH), but it has several limitations. Many centres use dual-colour in situ hybridisation (DISH) assays to detect HER2 gene amplification, which is cost less and can be done in a general laboratory. ...

Myoepithelial carcinoma, also known as malignant myoepithelioma, is mainly encountered in the salivary glands and, at a lower incidence, in the sweat glands (skin), breast, soft tissue, bone and very rarely in the lung. Pulmonary myoepithelial carcinoma was first described by Higashiyama et al. in 1998, and to the best of our knowledge, only 15 cases have been reported in the English literature so far....

ปัจจุบันนี้ได้มีการนำวิธีการด้านอณูพยาธิชีววิทยา (Molecular pathobiology) มาประยุกต์ใช้ร่วมกับวิธีอิมมูโนฮีสโตเคมี [Immunohistochemistry (IHC)] เพื่อการประเมินและวิเคราะห์ข้อมูลการแสดงออกของยีนบนพื้นฐานของวิธีแบบไมโครอาร์เรย์ (Microarray-based gene expression profiling)...

Low-grade central osteosarcoma accounts for 1-2% of all osteosarcomas, and is usually found in long bones of extremities. To our best knowledge, low-grade central osteosarcoma of pelvic bone is unusually rare....

Diffuse large B-cell lymphoma (DLBCL) can be found involving bone marrow in approximately 11-27% of cases 1. Patterns of involvement are variable; these include diffuse pattern (lymphomatous cells diffusely infiltrating the marrow), interstitial pattern (i.e. small amount of lymphomatous cells scattered among hematopoietic cells), nodular pattern (lymphomatous cells forming aggregates in intertrabecular or paratrabecular area of the marrow).. ...

Synovial sarcoma (SS) is a relatively common sarcoma of soft tissue arising in extremities of young adult, which is often diagnosed by histology and immunohistochemistry (IHC). Synovial sarcoma can be found in various locations and may cause diagnostic dilemma in tumors arising in unusual locations. In this study, we evaluated the application of...