[OA-09] Assessment of different cut-offs of equivocal HER2 IHC in breast cancer for HER2 ISH to reach appropriate sensitivity, AUC and reduction of ISH assay

The equivocal range of HER2 immunohistochemistry (IHC) is complete weak to moderate staining > 10% of breast cancer cells. This range has increased the numbers of HER2 in situ hybridisation (ISH) testing compared to the previous recommendations. ...

Author : Chayanan Puriwat, Natthawadee Laokolrat, Panitta Sitthinamsuwan, Malee Warnnissorn, Naree Warnnissorn, Norasate Samarnthai
Keyword : breast cancer, dual-colour in situ hybridisation, HER2, immunohistochemistry, percentage cut-off
Category : Brief communications

[OA-02] Concordance rate of HER2 immunohistochemistry compared to dual-colour in situ hybridisation (DISH) for detecting HER2 amplification in breast cancer

Gold standard of HER2 amplification detection is fluorescence in situ hybridisation (FISH), but it has several limitations. Many centres use dual-colour in situ hybridisation (DISH) assays to detect HER2 gene amplification, which is cost less and can be done in a general laboratory. ...

Author : Adiluck Pisutpunya, Kroonpong Iampenkhae
Keyword : dual-colour in situ hybridisation, gene amplification, HER2 status, immunohistochemistry
Category : Brief communications

A Comparison Study of Molecular Classification of Primary Invasive Breast Carcinoma and Corresponding Metastatic Carcinoma in Axillary Lymph Nodes by Immunohistochemistry

Breast cancer is a heterogeneous disease. There is a high degree of diversity between and within tumours as well as among cancer-bearing individuals, and all of these factors together determine the risk of disease progression and therapeutic resistance....

Author : Tanyasorn Sutthipongsupa, Kittisak Wongchansom, Panjawit Wuttipanich, Mongkon Charoenpitakchai
Keyword : Breast cancer, heterogeneity, molecular phenotypes, paired node, ER, PR, HER2, Ki67, CK5/6, EGFR, p53
Category : Original Articles